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KMID : 0900919970210020177
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Korean journal of Animal Reproduction
1997 Volume.21 No. 2 p.177 ~ p.183
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Effect of Epidermal Growth Factor and Transforming Growth Factor-alpha on In Vitro Maturation of Porcine Oocytes
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Park Byung-Kwon
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Abstract
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The present study examined the effects of epidermal growth factor(EGF) and transforming growth factor-alpha(TGF-alpha) on in vitro maturation of porcine follicular oocytes. Basic medium used TCM-HEPES, and oocytes cultured for 42 hours in vitro. The results obtained are as follows; 1. The nuclear maturation rates of EGF-treated groups(10ng/ml, 75.9% ; 30ng/ml, 69.2% ; 50ng/ml, 67.2% ; 100ng/ml, 71.0%) on the porcine oocytes cultured in medium without pFF in vitro were significantly(P<0.01) higher than those of non-treated group(57.1%). When the oocytes were cultured in media with 10%(v/v) pFF, the nuclear maturation rates of 30ng EGF/ml(77.1%) treated group were significantly(P<0.01) higher than those of non-(59.2%) and EGF-treated groups(10ng/ml, 65.4% ; 50ng/ml, 65.5% ; 100ng/ml, 70.4%). 2. The nuclear maturation rates of 30ng TGF-alpha/ml treated group(71.9%) in media without pFF in vitro were significatnly(P<0.01) higher than those of non-(57.1%) and TGF-alpha treated groups(10ng/ml, 60.4% ; 50ng/ml, 65.4% ; 100ng/ml, 60.0%). When the oocytes were cultured in media with 10%(v/v) pFF, the nuclear maturation rates of 30 and 50ng TGF-alpha/ml(77.4% and 79.6%) treated groups(10ng/ml, 64.2% ; 100ng/ml, 61.6%). 3. On the effect of EGF(30ng/ml) and/or TGF-alpha(30ng/ml) treated groups in medium without pFF in vitro, the nuclear maturation rates indicated 57.3, 60.4, 75.9 and 79.7% in media with no EGF & TFG-alpha, TGF-alpha only, EGF only nad EGF＋TGF-alpha treated groups, respectively. The nuclear maturation rates in medium with EGF only or EGF＋TGF-alpha were significantly(P<0.01) higher than those non- and TGF-alpha treated groups. When the oocytes were cultured in media with 10%(v/v) pFF, the nuclear maturation ratesof EGF＋TGF-alpha treated group(75.9%) were significantly(P<0.01) higher than those of non-(59.2%), TGF-alpha only (64.2%) and EGF only(69.4%) treated groups.
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KEYWORD
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Porcine oocyte, In vitro maturation, Growth factor, EGF, TGF-alpha
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